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KMID : 0545120000100020267
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 2 p.267 ~ p.271
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Characterization of Two Glucans Activating an Alternative Complement Pathway from the Fruiting Bodies of Mushroom Pleurotus ostreatus
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KWEON, MEE-HYANG
LIM, WANG-JIN/YANG, HAN-CHUL/SUNG, HA-CHIN
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Abstract
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Two glucans (PONGa and PONGb) differing in their anomeric and glycosidic linkage structures were isolated from the water-insoluble materials (PON) of Pleurotus ostreatus basidiocarps, which activated the complement system and were almost soley composed of D-glucose. The isolation was achieved by repeated precipitations with ethanol and adsorption on concanavalin A (Con A) of PON suspension in thy㏖/NaCl. Based on methylation analysis, IR, GLC-MS, ^1H, and ^13C-NMR spectroscopies, PONGa was found to be a branched α-glucan composed of α-1,3-linked D-glucopyranose residues and α-1,3-linked units with 6branching points, whereas PONGb was a linear β-glucan composed mainly of β-1,3-linked D-glucopyranose residues. The PONGb particles reacted more potently than the PONGa particles as C3 activator in alternative complement hemolysis and crossed-immunoelectrophoresis using anti-human C3, thereby suggesting that the complement activating components of PON were β-(13)-glucans rather than α-glucan components.
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